ASTM Biodiesel Specifications

These tables show selected ASTM requirements for B100 and B6 to B20. Note that the specifications are routinely updated and modified. Check with your fuel provider to ensure they are storing biodiesel meeting the most recent fuel quality standards. To learn more about biodiesel, go to the Fuel Basics or Blends pages. The National Biodiesel Accreditation Program takes fuel quality a step further with the BQ9000 program combining ASTM standards and a quality systems program, which includes biodiesel production through end-use.


This table shows selected requirements for B100, 100% biodiesel, as listed in ASTM D6751-15a. The full standard can be purchased from ASTM International.

PropertyTest MethodGrade No.1-B S15Grade No.1-B S500Grade No.2-B S15Grade No.2-B S500
Sulfur, % mass (ppm), maxD54530.0015 (15)0.05 (500)0.0015 (15)0.050 (500)
Cold soak filterability, s, maxD7501200200360360
Monoglycerides, % mass, maxD65840.40.4--
Requirements for All Grades
Calcium and Magnesium, combined, ppm, maxEN145385555
Flash point (closed cup), °C, minD9393939393
Alcohol Control:
One of the following must be met
(1) Methanol content, mass%, maxEN141100.
(2) Flash point, °C, minD93130130130130
Water and sediment, % volume, maxD27090.
Kinematic viscosity, mm2/s, 40°CD4451.9-6.01.9-6.01.9-6.01.9-6.0
Sulfated ash, % mass, maxD8740.
Copper strip corrosionD130No. 3No. 3No. 3No. 3
Cetane number, minD61347474747
Cloud point, °CD2500ReportReportReportReport
Carbon residuea, % mass, maxD45300.
Acid number, mg KOH/g, maxD6640.
Free glycerin, % mass, maxD65840.
Total glycerin, % mass, maxD65840.
Phosphorus content, % mass, maxD49510.0010.0010.0010.001
Distillation temperature, 90% recovered (T90)b, °C, maxD1160360360360360
Sodium and potassium, combined, ppm, maxEN145385555
Oxidation stability, hrs, minEN157513333

a Carbon residue shall be run on the 100% sample.
b Atmospheric equivalent temperature.

B6 to B20

This table shows requirements for 6% biodiesel (B6) to 20% biodiesel (B20) as listed in ASTM D7467-13. The full standard can be purchased from ASTM International.

PropertyTest MethodGrade
B6 to B20 S15 B6 to B20 S500 j B6 to B20 S5000
Acid number, mg KOH/g, max.D6640.30.30.3
Viscosity, mm2/s at 40°CD4451.9-4.1a1.9-4.1a1.9-4.1a
Flash point, °C, minD9352b52b52b
Cloud point, °C, max or LTFT/CFPP, °C, maxD2500ccc
Sulfur Content, (μg/g or ppm)
 mass %, max.
 mass %, max.


Distillation temperature, °C, 90% evaporated, maxD86343343343
Ramsbottom carbon residue on 10% bottoms, mass %, max.D5240.350.350.35
Cetane number, minD613404040
One of the following must be met:
(1) Cetane index, minD976-80404040
(2) Aromaticity, vol %, max.D1319-033535
Ash Content, mass %, max.D4820.010.010.01
Water and Sediment, vol %, max.D27090.050.050.05
Copper Corrosion, 3 h @ 50°C, maxD130No. 3No. 3No. 3
Biodiesel Content, % (V/V)D73716.–20.6.–20.6.–20.
Oxidation Stability, hours, min.EN15751666
Lubricity, HFRR @ 60°C, (micron μm), max.D6079520520520

a If Grade No. 1-D or blends of Grade No. 1-D and Grade No. 2-D diesel fuel are used, the minimum viscosity shall be 1.3 mm2/s.
b If Grade No. 1-D or blends of Grade No. 1-D and Grade No. 2-D diesel fuel are used, or a cloud point of less than -12°C) is specified, the minimum flash point shall be 38°C.
c It is unrealistic to specify low-temperature properties that will ensure satisfactory operation at all ambient conditions. However, satisfactory operation below the cloud point (or wax appearance point) may be achieved depending on equipment design, operating conditions, and the use of flow-improver additives as described in X3.1.2. Appropriate low-temperature operability properties should be agreed upon between the fuel supplier and purchaser for the intended use and expected ambient temperatures. Test Methods D 4539 and D 6371 may be useful to estimate vehicle low temperature operability limits when flow improvers are used, but their use with Bxx blends from a full range of biodiesel feedstock sources has not been validated. Due to fuel delivery system, engine design, and test method differences, low-temperature operability tests may not provide the same degree of protection in various vehicle operating classes. Tenth percentile minimum air temperatures for U.S. locations are provided in Appendix X3 as a means of estimating expected regional temperatures. The tenth percentile minimum air temperatures may be used to estimate expected regional target temperatures for use with Test Methods D 2500, D 4539, and D 6371. Refer to X3.1.3 for further general guidance on test application.